Coding

Part:BBa_J56002:Design

Designed by: Matt Eames   Group: iGEM06_UCSF   (2006-10-13)


Tar EA400 has orthogonal interaction with CheW VM108


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 181
    Illegal PstI site found at 1304
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1456
    Illegal PstI site found at 181
    Illegal PstI site found at 1304
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1663
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 181
    Illegal PstI site found at 1304
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 181
    Illegal PstI site found at 1304
    Illegal NgoMIV site found at 1605
    Illegal AgeI site found at 1282
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Mutation was originally evolved in Tsr (EA402), this residue occurs in position 400 after sequence alignment. Designed to interact with part BBa_J56001. Includes XhoI restriction site at terminus.

Source

Synthesized by DNA 2.0

References

Liu et al, Journal of Bacteriology (1991)